| 黄石伟,黄婷,张凌瑞,邱海吉,谢涵涵,徐炯志,贾海锋.多肽调控乙酰化水平促进荔枝果实着色[J].中国南方果树,2026,55(4):94-101 |
| 多肽调控乙酰化水平促进荔枝果实着色 |
| The Polypeptide Regulates Acetylation Levels to promote Litchi Fruit Coloring |
| 投稿时间:2026-02-11 修订日期:2026-05-14 |
| DOI: |
| 中文关键词: 荔枝 果实色泽 乙酰化 去乙酰化;花色苷代谢 |
| 英文关键词:Lichi fruit color acetylation deacetylation anthocyanin metabolism |
| 基金项目:广西科技基地和人才专项—亚热带国重室项目”(桂科AD25069107); 国家现代农业产业技术体系广西荔枝龙眼创新团队(nycytxgxcxtd-2023-12-01) |
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| 中文摘要: |
| 【目的】为了分析多肽对荔枝果树发育的影响,以‘钦州红荔’荔枝为试材,分别采取S1、S2和S3时期的荔枝果实进行多肽处理,对果实发育期间的品质指标进行了研究。【方法】通过测定果实大小、糖酸等生理指标和与果实花色苷合成相关的基因表达量,以及多肽对乙酰化水平的影响。【结果】表明,多肽促进了荔枝果实的着色,在荔枝果实发育的S1、S2和S3时期类黄酮含量均显著上升。对花色素苷生物合成相关基因进行探究,发现LcPAL等3个结构基因和LcMYB1等2个调控基因表达显著升高,并且抑制大部分去乙酰化基因的表达,同时促进了大部分乙酰化基因的表达。Western Blot检测发现,在多肽处理后,LcHDT8的乙酰化水平先降低后升高,LcHAG2的乙酰化水平则先升高后降低。【结论】综上所述,多肽可以调控荔枝果实的乙酰化水平,进而促进花色苷积累,提升果实品质。 |
| 英文摘要: |
| [Objective] To analyze the impact of peptides on the development of lychee trees, ''Qinzhou Red Lychee'' was used as the test material, and lychee fruits at the S1, S2, and S3 stages were treated with peptides. The quality indicators during fruit development were studied. [Methods] The study measured fruit size, sugar and acid content, and the expression levels of genes related to anthocyanin synthesis in the fruit, as well as the effect of peptides on acetylation levels. [Results] The results showed that peptides promoted the coloring of lychee fruits, with significant increases in flavonoid content during the S1, S2, and S3 stages of fruit development. Exploration of genes related to anthocyanin biosynthesis revealed that the expression of three structural genes, including LcPAL, and two regulatory genes, including LcMYB1, significantly increased, while inhibiting the expression of most deacetylation genes and promoting the expression of most acetylation genes. Western Blot analysis showed that after peptide treatment, the acetylation level of LcHDT8 first decreased and then increased, while the acetylation level of LcHAG2 first increased and then decreased. [Conclusion] In summary, peptides can regulate the acetylation level of lychee fruits, thereby promoting anthocyanin accumulation and enhancing fruit quality. |
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