| 刘慧超,吴小波,赵建设,赵致钧.外源谷氨酸处理对草莓组培瓶苗驯化生长和繁苗率的影响[J].中国南方果树,2026,55(4):194-197 |
| 外源谷氨酸处理对草莓组培瓶苗驯化生长和繁苗率的影响 |
| Effects of Exogenous Glutamic Acid Treatment on Acclimatization Growth and Seedling Propagation Rate of Strawberry Tissue Culture Plantlets in Vitro |
| 投稿时间：2025-11-04 修订日期：2025-12-06 |
| DOI： |
| 中文关键词: 草莓 组培瓶苗 谷氨酸 驯化 繁苗率 |
| 英文关键词:Strawberry In vitro tissue-cultured plantlets Glutamic acid Acclimatization Seedling propagation rate |
| 基金项目:河南省科技攻关项目 |
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| 中文摘要: |
| 摘要：以‘郑莓一号’草莓脱毒组培瓶苗为试材，研究了营养液中添加外源谷氨酸处理对其瓶外驯化生长及定植后繁苗率的调控效应。结果表明：与对照(CK 0mmol/L 谷氨酸)相比，不同浓度谷氨酸处理存在显著剂量效应。研究发现，1.0mmol/L 谷氨酸(T2)处理效果最优，瓶外驯化生长期，苗鲜重、干重、株高、茎粗、叶片厚度分别较 CK 提高 8.57%、8.34%、16.18%、15.28%、28.64%；SPAD 值与叶片氮含量分别较 CK 提升21.93%、21.80%；定植后繁苗率较 CK 显著提高36.52%（P<0.05)）；0.5mmol/L 谷氨酸(T1)处理对苗驯化生长及繁苗率有一定促进作用(繁苗率提高14.23%），1.5mmol/L 谷氨酸(T3)处理与 CK无显著差异（繁苗率提高3.99%）。本试验条件下，1.0mmol/L 外源谷氨酸处理可显著促进‘郑莓一号’草莓组培瓶苗驯化生长及繁殖能力，可为草莓脱毒种苗产业化育苗提供技术参考。 |
| 英文摘要: |
| Abstract
Taking virus-free tissue culture plantlets of ‘Zhengmei No.1’ strawberry as the test materials, this study investigated the regulatory effects of exogenous glutamic acid added to nutrient solution on their ex vitro acclimatization growth and seedling propagation rate after transplanting. The results showed that compared with the control (CK, 0 mmol/L glutamic acid), different concentrations of glutamic acid treatments exhibited a significant dose effect. It was found that the treatment with 1.0 mmol/L glutamic acid (T2) achieved the optimal effect. During the ex vitro acclimatization period, the fresh weight, dry weight, plant height, stem diameter and leaf thickness of the plantlets in T2 were increased by 8.57%, 8.34%, 16.18%, 15.28% and 28.64% respectively compared with those in CK; the SPAD value and leaf nitrogen content were increased by 21.93% and 21.80% respectively compared with CK; the seedling propagation rate after transplanting was significantly increased by 36.52% compared with CK (P<0.05). The treatment with 0.5 mmol/L glutamic acid (T1) had a certain promoting effect on seedling acclimatization growth and propagation rate (the propagation rate was increased by 14.23%), while the treatment with 1.5 mmol/L glutamic acid (T3) showed no significant difference from CK (the propagation rate was increased by 3.99%). Under the conditions of this experiment, the treatment with 1.0 mmol/L exogenous glutamic acid could significantly promote the acclimatization growth and reproductive capacity of ‘Zhengmei No.1’ strawberry tissue culture plantlets, which can provide technical reference for the industrial seedling raising of virus-free strawberry seedlings. |
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