| 肖利凤.健康与患病蓝莓植株根际微生物组差异研究[J].中国南方果树,2026,55(2): |
| 健康与患病蓝莓植株根际微生物组差异研究 |
| Comparative Analysis of the Rhizosphere Microbiome Between Healthy and Diseased Blueberry Plants |
| 投稿时间：2025-09-30 修订日期：2025-10-20 |
| DOI： |
| 中文关键词: 蓝莓 炭疽菌 根际微生物组 16S rRNA ITS |
| 英文关键词:blueberry Colletotrichum?spp. rhizosphere microbiome 16S rRNA ITS |
| 基金项目:凯里学院省级名师杨芩工作室（MSGZS-SJ-2024002）；凯里学院博士专项基金（No.BS20240216）；贵州省特色园艺作物分子育种重点实验室 [2025] 027。 |
|
| 摘要点击次数: 15 |
| 全文下载次数: 0 |
| 中文摘要: |
| 【【目的】南高丛蓝莓'薄雾'因其高产性和适应性被广泛种植。然而，该品种在田间对炭疽病的抗性表现存在显著差异。本研究旨在评估炭疽菌诱发的植株病变对蓝莓根际细菌及真菌微生物群落的影响；揭示健康植株与患病植株根际微生物群落的差异特征，为蓝莓根际微生物组提供了全面视角。【方法】本研究采用16S rRNA和ITS测序技术，对健康与患病的南高丛蓝莓'薄雾'植株的根际微生物群落进行多样性分析与功能差异评估。【结果】研究发现'薄雾'根际微生物群落α多样性与β多样性均存在显著差异，健康根际比病株根际拥有更多独特的细菌和真菌ASV。健康根际中假单胞菌Pseudomonas、伯克霍尔德菌Burkholderiales和噬几丁质菌Chitinophagales相对丰度显著升高；而病株根际则富集放线菌Actinobacteriota、根瘤菌Rhizobiales、酸杆菌Acidobacteriaceae等细菌群落，以及毛舌菌Trichoglossum和假丝孢菌Sarocladium等真菌群落。炭疽病的严重发生诱导了根际细菌与真菌群落组成发生转变，尤其显著影响核心微生物组成分的相对丰度，其变化幅度显著，患病植株根际微生物群落组成较健康根际更为复杂。【结论】在同质条件下，暴露于病原体的植株要么保持健康，要么严重发病，这种结果很可能受到微生物群落调控的影响，而根际微生物群落的组成或功能差异可能直接影响病害发展进程。 |
| 英文摘要: |
| 【Objective】The highbush blueberry cultivar 'Misty' is extensively cultivated due to its high productivity and adaptability. Nevertheless, field resistance to anthrax disease exhibits considerable variability among individual plants. This study seeks to assess the impact of anthracnose-induced branch dieback on the bacterial and fungal communities within the blueberry rhizosphere. It aims to elucidate the differential characteristics of rhizosphere microbial communities between healthy and diseased plants, and provide a comprehensive perspective on the blueberry rhizosphere microbiome. 【Methods】Employing 16S rRNA and ITS sequencing technologies, diversity analysis and functional differential assessment were performed on the rhizosphere microbial communities of healthy and diseased 'Misty' highbush blueberry plants. 【Results】The study identified significant variations in both α-diversity and β-diversity among the root-associated microbial communities of 'Misty'. The rhizosphere of healthy plants contained a greater number of unique bacterial and fungal amplicon sequence variants (ASVs) compared to that of diseased plants. Notably, the relative abundances of Pseudomonas, Burkholderiales, and Chitinophagales were significantly higher in the rhizosphere of healthy plants. In contrast, the rhizosphere of diseased plants showed an enrichment of bacterial communities such as Actinobacteriota, Rhizobiales, and Acidobacteriaceae, as well as fungal communities including Trichoglossum and Sarocladium. The severe occurrence of anthrax led to notable shifts in the composition of rhizosphere bacterial and fungal communities, particularly influencing the relative abundance of core microbial constituents. These alterations were pronounced, with the microbial communities in the rhizosphere of diseased plants displaying greater complexity than those in healthy plants.【Conclusion】Under uniform environmental conditions, plants exposed to pathogens either remain healthy or succumb to severe disease. This dichotomy is likely mediated by the regulation of microbial communities, suggesting that variations in the composition or function of the rhizosphere microbial community may have a direct impact on disease progression. |
| 查看/发表评论 下载PDF阅读器 |
| 关闭 |
