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余谦,王德欢,朱明媛,张昭阳,祝菊红,梁欢,胡江勇,施先锋,葛米红,宋晶.链霉菌JKTJ-3固体发酵及其对草莓母苗促生作用研究[J].中国南方果树,2026,55(4):177-184
链霉菌JKTJ-3固体发酵及其对草莓母苗促生作用研究
Study on solid-state fermentation of?Streptomyces?JKTJ-3 and its growth-promoting effects on strawberry mother seedlings
投稿时间:2025-09-18  修订日期:2025-11-09
DOI:
中文关键词:  链霉菌  固体发酵  草莓母苗  促生作用  促扩繁
英文关键词:Streptomyces  solid-state fermentation  strawberry mother seedling  growth- promoting  promote propagation
基金项目:湖北省科技创新人才计划项目“设施草莓集约化高效育苗关键技术研究与示范”(2023DJC118);湖北省农业农村领域公益事业类青年科学家项目“草莓设施立体栽培技术装备及模式应用研究与示范”(2023BBB041);武汉市科技计划项目“草莓基质育苗高效扩繁技术研究”(2023110201030684)资助。
作者单位E-mail
余谦 武汉市农业科学院 gmh917@126.com 
王德欢 武汉市农业科学院 zmwdh1986@163.com 
朱明媛 武汉市农业科学院 1257271428@qq.com 
张昭阳 武汉市农业科学院 95359632@qq.com 
祝菊红 武汉市农业科学院 zmzjh1986@163.com 
梁欢 武汉市农业科学院 904873454@qq.com 
胡江勇 武汉市农业科学院 2931043305@qq.com 
施先锋 武汉市农业科学院 zmsxf1981@163.com 
葛米红* 武汉市农业科学院 gmh917@126.com 
宋晶 武汉市农业科学院 41861099@qq.com 
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中文摘要:
      采用单因素实验法,以麦麸、草炭和蚯蚓粪等为基质,对链霉菌JKTJ-3的固体发酵培养基及发酵条件进行筛选与优化。通过接种JKTJ-3菌剂,比较不同接种量对草莓母苗生长的影响。结果表明,最佳发酵培养基组成为草炭与麸皮(3:2,W/W);添加2%(W/W)甘油作为碳源,基质总量20 g/250 mL,料水比为1:1.6(W/V),接种3块菌块,于35℃发酵7 d,孢子量最高达4×1011 CFU/g。JKTJ-3菌剂可促进草莓母苗生长、养分积累和扩繁,不同接种量在不同时期表现存在差异,其中3 g/L接种处理的促生综合表现最佳。
英文摘要:
      A single-factor experimental approach was employed to screen and optimize the solid-state fermentation medium and conditions for?Streptomyces?JKTJ-3 using substrates such as wheat bran, peat, and vermicompost. By inoculating with JKTJ-3 agent, the effects of different inoculation rates on the growth of strawberry mother seedlings were compared. The results showed that the optimal fermentation medium consisted of peat and wheat bran at a ratio of 3:2 (W/W), with the addition of 2% (W/W) glycerol as carbon source. The total substrate amount was 20 g/250 mL, the material-to-water ratio was 1:1.6 (W/V), and inoculation was performed with 3 agar plugs of JKTJ-3, followed by fermentation at 35°C for 7 days. Under these optimized conditions, the maximum spore count reached 4 × 1011 CFU/g. The JKTJ-3 agent promoted the growth, nutrient accumulation, and propagation of strawberry mother seedlings. The effects varied with inoculation rate and growth period, with the 3 g/L inoculation treatment showing the best overall growth-promoting performance.
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