| 宋兆伟,赵新红,崔瀚元,张宇微,张越,丁舒,陈晓明,陈颖.复合酶辅助水提富士苹果幼果多酚的抗氧化活性评价[J].中国南方果树,2026,55(4):163-169 |
| 复合酶辅助水提富士苹果幼果多酚的抗氧化活性评价 |
| Antioxidant activity of polyphenols from young fruits of Fuji apple extracted with the assistance of complex enzymes |
| 投稿时间：2025-09-08 修订日期：2025-10-15 |
| DOI： |
| 中文关键词: 富士幼果 苹果多酚 抗氧化活性 复合酶水提 自由基 |
| 英文关键词:young fuji apple fruit apple polyphenols antioxidant activity compound enzyme water extraction free radical |
| 基金项目:甘肃省东西部科技协作专项(津甘合作、鲁甘合作)项目(24CXNE012)资助。 |
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| 中文摘要: |
| 由于提取过程完全不使用有机溶剂，复合酶辅助水提法是一种绿色环保的富士幼果多酚制备工艺。为富士幼果的资源化利用和食品产品开发提供理论依据和技术参考，本研究采用了6种体外抗氧化评价体系，评价了复合酶辅助水提法制备的富士幼果多酚粗提物的抗氧化活性。结果表明，该工艺制备的富士幼果多酚粗提物对DPPH·、ABTS+·和O- 2·的清除作用优于醇提幼果多酚，且对DPPH·、O- 2·、·OH和NO- 2·的清除作用优于醇提苹果渣多酚，但对FRAP的还原能力较弱。幼果多酚粗提物浓度与各抗氧化指标之间均存在显著（P＜0.05）的正相关，其强弱顺序为：DPPH·＞ABTS+·＞NO- 2·＞O- 2·＞·OH＞FRAP，对应的最大清除率和还原能力分别为：96.05±0.10%、100.00±0.09%、94.12±1.60%、82.74±2.79%、57.41±1.11%和0.60±0.01 OD700。综上所述，复合酶辅助水提的富士苹果幼果多酚粗提物具有较强的体外抗氧化活性，作为一种天然抗氧化剂具有广阔的开发和应用前景。 |
| 英文摘要: |
| The compound enzyme-assisted water extraction method, which does not utilize organic solvents, represents a green and environmentally friendly approach for preparing polyphenols from Fuji young fruits. To establish a theoretical foundation and provide technical guidance for the resource utilization and food product development of Fuji young fruit, six antioxidant evaluation systems were employed to assess the antioxidant activity of the polyphenol crude extract obtained through this extraction method. The results indicated that the scavenging activity of polyphenols extracted from Fuji young fruit surpassed that of polyphenols extracted using alcohol, as measured by DPPH·, ABTS+·, and O- 2·assays. Furthermore, the scavenging activity of alcohol-extracted polyphenols from Fuji young fruit on DPPH·, O- 2·, ·OH, and NO- 2· was superior to that of polyphenols extracted from apple pomace using alcohol; however, the reducing ability of alcohol-extracted polyphenols from Fuji young fruit was weak in the FRAP assay. A significant positive correlation was observed between polyphenol concentration and antioxidant indices (P<.05), with the following order of efficacy: DPPH· > ABTS+· > N O- 2· > O- 2· > ·OH > FRAP. The corresponding maximum scavenging rates and reducing capacities were 96.05±0.10%, 100.00±0.09%, 94.12±1.60%, 82.74±2.79%, 57.41±1.11%, and 0.60±0.01 OD700, respectively. In conclusion, polyphenols extracted from immature Fuji apple fruit using complex enzyme-assisted water extraction exhibit significant in vitro antioxidant activity and hold considerable potential for development and application as a natural and environmentally friendly antioxidant. |
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