| 李海炎.140份桃种质资源细菌性穿孔病抗性评价及叶片微观特征观察[J].中国南方果树,2026,55(4):232-237 |
| 140份桃种质资源细菌性穿孔病抗性评价及叶片微观特征观察 |
| Evaluation of Resistance to Peach Bacterial Shot Hole and Observation of Microscopic Leaf Characteristics of 140 Peach Germplasm Resources |
| 投稿时间：2025-08-27 修订日期：2025-10-21 |
| DOI： |
| 中文关键词: 桃 细菌性穿孔病 Xap 抗性鉴定 气孔 |
| 英文关键词:Peach Bacterial shot hole Xap Resistance identification Stomata |
| 基金项目:国家自然科学基金项目 |
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| 中文摘要: |
| 针对桃细菌性穿孔病在我国各桃主产区危害呈现逐年上升的趋势,对140份桃种质资源进行Xap抗性鉴定评价,以筛选抗性种质,为抗病育种提供参考。本研究采用注射接种法在生长植株叶片上进行活体接种,15天后调查病情指数并划分抗性等级；对高抗、高感种质资源叶片进行扫描电镜观察叶片表皮和气孔结构特征。结果表明：有19份高抗(HR)种质(占13.57%)、52份抗病(R)种质(占37.14%)、28份中抗(MR)种质(占20.00%)、23份感病(S)种质(占16.43%)以及18份高感(HS)种质(占12.86%)；扫描电镜观察抗病和感病种质的叶片结构特征发现,感病种质叶片气孔中有大量Xap病原菌聚集,Xap可以通过气孔侵染叶片；接种Xap后,引起叶片气孔变小。 |
| 英文摘要: |
| With increasing incidence of peach bacterial shot hole in peach producing areas, 140 peach germplasm resources were identified for Xap resistance and evaluated for disease resistance breeding. In this study, an injection inoculation method was used to identify inoculation in growing plants leaves, analyze disease indexes, and classify resistance levels. The leaf epidermis and stomatal structure were studied using a scanning electron microscope. The results of Xap resistance identification of 140 peach germplasm resources showed: There were 19 highly resistant (HR) germplasm (13.57%), 52 resistant (R) germplasm (37.14%), 28 moderately resistant (MR) germplasm (20.00%), 23 susceptible (S) germplasm (16.43%), and 18 highly susceptible (HS) germplasm (12.86%). By comparing the leaf structural features of susceptible and resistant varieties, it was shown that Xap could infect leaves through stomata and that the susceptible variety"s leaves contained large amounts of Xap in their stomata. The stomata on the leaves smaller following Xap inoculation. |
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