| 陈莹,罗健铭,赵和国,张林坤,蔡楠,张凤,韩健,曹运琳,马先锋.沙田柚×宜昌橙F1杂种群体创建及遗传性状分析[J].中国南方果树,2026,55(3): |
| 沙田柚×宜昌橙F1杂种群体创建及遗传性状分析 |
| Establishment of Citrus maxima × Citrus ichangensis . hybrid population and Analysis of genetic traits |
| 投稿时间：2025-06-07 修订日期：2025-06-30 |
| DOI：10.13938/j.issn.1007-1431.20250265 |
| 中文关键词: 沙田柚 宜昌橙 杂交后代 分子鉴定 遗传分析 |
| 英文关键词:Citrus maxima Citrus ichangensis hybrid offspring molecular identification genetic analysis |
| 基金项目:国家自然科学基金联合基金；岳麓山实验室联合引进人才项目 |
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| 中文摘要: |
| 以沙田柚为母本与宜昌橙为父本,获得性状分离的杂种后代,完成叶形态学性状遗传分析,为优良性状的克隆提供丰富的群体资源。本研究构建沙田柚×长安基地宜昌橙XJY-1杂交群体,结合InDel分子标记与形态学方法对385株F?单株进行鉴定及遗传分析。InDel分子标记结果表明,所有杂交植株均为真实杂交后代。F1植株叶片与亲本叶片作遗传分析的形态学分析显示,其全叶长、叶宽、翼叶长及翼叶宽的变异系数为21.50%~35.01%,翼叶宽变异系数最大,为35.01%,表明翼叶宽离散程度较高,分布较广；全叶长变异系数最小,为21.50%。上述性状的杂种优势指数均高于1,表明其受遗传因素主导,且呈现趋中遗传。翼叶长/本叶长、叶面积、翼叶面积和翼叶面积/本叶面积等性状的变异系数范围为14.73%~57.55%,其中翼叶面积变异系数最高,为57.55%；除翼叶面积/本叶面积的杂种优势最低,为94.49%,其他性状的杂种优势均大于1。沙田柚与宜昌橙杂交苗均为杂种后代且叶片形态性状表现出丰富的遗传变异,具有明显的趋中遗传和杂种优势。 |
| 英文摘要: |
| Using Shatian pummelo (Citrus maxima) as the female parent and Chang"an Base XJY-1(Citrus ichangensis) as the male parent, hybrid progeny with trait segregation were generated, and genetic analysis of leaf morphological traits was conducted to provide abundant population resources for cloning superior traits. In this study, a hybrid population of C. maxima × C. ichangensis was established. A total of 385 F? individuals were identified and genetically analyzed using a combination of InDel molecular markers and morphological methods. Results from InDel molecular marker analysis confirmed that all hybrid plants were true hybrid offspring. Morphological and genetic analyses of leaves from F? plants and their parents revealed that the coefficients of variation (CVs) for total leaf length, leaf width, wing leaf length, and wing leaf width ranged from 21.50% to 35.01%. Among these, wing leaf width exhibited the highest CV (35.01%), indicating a high degree of dispersion and broad distribution, while total leaf length had the lowest CV (21.50%). Heterosis indices (HIs) for all above-mentioned traits exceeded 1, suggesting that these traits were predominantly governed by genetic factors and displayed centralized inheritance. For traits including wing leaf length/lamina length, leaf area, wing leaf area, and wing leaf area/lamina area, CVs ranged from 14.73% to 57.55%, with wing leaf area showing the highest CV (57.55%). Except for the wing leaf area/lamina area trait, which had a lower HI (94.49%), all other traits exhibited HIs greater than 1. Collectively, these results demonstrate that hybrid seedlings of C. maxima and C. ichangensis are authentic hybrid progeny, with leaf morphological traits showing abundant genetic variation and evident centralized inheritance as well as heterosis. |
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