| 蒋雨含,晏重西,李勋兰,彭芳芳,韩国辉,魏召新.离体柠檬茎段热处理抑制黄脉病病毒效果研究简报+增刊[J].中国南方果树,2025,54(S1): |
| 离体柠檬茎段热处理抑制黄脉病病毒效果研究简报+增刊 |
| Brief Study on the Effect of Heat Treatment of Isolated Lemon Stem Segments on Inhibiting Yellow Vein Disease Virus |
| 投稿时间：2025-06-04 修订日期：2025-08-18 |
| DOI： |
| 中文关键词: 柠檬 离体培养 热处理 病毒 |
| 英文关键词:lemon in vitro culture heat treatment virus |
| 基金项目:重庆市农业科学院绩效激励项目（cqaas2021jxj107）, 重庆市技术创新与应用发展专项（CSTB2023TIAD-KPX0044） |
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| 中文摘要: |
| 热处理是柠檬脱毒及抗耐性研究中一种重要方法，离体培养能够克服环境影响，缩短培育周期，提高繁殖系数。对离体条件下的尤力克柠檬茎段开展了热处理的温度和时长试验，并以为害尤力克柠檬最为严重的黄化脉明病毒（Citrus yellow vein clearing virus, CYVCV）为对象，对热处理抑制病毒的效果进行了实时定量检测。结果表明,随着热处理温度的升高，离体茎段组织结构得到不同程度的破坏，电导率升高。51℃处理30min离体茎段能够正常发育，病毒含量得到明显抑制，是离体尤力克柠檬茎段热处理的极端耐受条件。 |
| 英文摘要: |
| Heat treatment is an important method in the study of detoxification and resistance of lemons. In vitro culture can overcome environmental influences, shorten the cultivation cycle and increase the reproduction coefficient. Temperature and duration experiments of heat treatment were carried out on the stem segments of Eurek lemon under in vitro conditions. Taking the Citrus yellow vein clearing virus (CYVCV), which is the most severely damaging to Eurek lemon, as the object, the effect of heat treatment on inhibiting the virus was quantitatively detected in real time. The results show that with the increase of heat treatment temperature, the tissue structure of the isolated stem segments is damaged to varying degrees, and the electrical conductivity increases. The isolated stem segments treated at 51℃ for 30 minutes can develop normally, and the virus content is significantly inhibited. This is an extreme tolerance condition for the heat treatment of isolated Yulike lemon stem segments. |
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