| 王志凯,朱允驰,邓会栋,乔阳,吴晓慧,郭利军,孙阳阳,钟义旺,叶迎君,黄海杰,冯学杰.四个榴莲品种不同贮藏条件下花粉活力及柱头可授性研究[J].中国南方果树,2026,55(4):124-129 |
| 四个榴莲品种不同贮藏条件下花粉活力及柱头可授性研究 |
| Comparative Analysis of Pollen Viability and Stigma Receptivity in Four Durian Varieties Across Different Storage Regimes |
| 投稿时间：2025-04-09 修订日期：2025-05-16 |
| DOI： |
| 中文关键词: 榴莲 贮藏温度 花粉生活力 MTT染色法 柱头可授性 |
| 英文关键词:Durio zibethinus storage temperature pollen viability MTT staining method stigma receptivity |
| 基金项目:2024年海南省重点研发项目“三亚榴莲品质控制技术研究与示范”(ZDYF2024XDNY251)；2025年海南省农业科学院基本科研业务费团队项目(ITFT2025TDYD01)；2024年海南省农业科学院基本科研业务费项目“榴莲花果代谢调控网络数据集的构建”(2024SJZD02)。 |
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| 中文摘要: |
| 本研究以四个不同榴莲品种作为试验材料,探讨不同榴莲品种间花粉活力及柱头可授性的强弱。通过联苯胺-过氧化氢法对柱头可授性进行测定,并利用MTT染色法测定了在室温(25 ℃)、4 ℃和-20 ℃等不同贮藏条件下榴莲花粉的活性变化。研究结果表明：榴莲柱头活性在花期的不同阶段呈现显著的时间性波动性和规律性。不同榴莲品种的柱头活性存在显著差异,其中‘金枕’的柱头活性显著高于其他三个品种。在花粉贮藏方面,室温条件下花粉活力衰退速度较快,且随着贮藏时间的延长,花粉活力显著下降；而低温贮藏(4 ℃和-20 ℃)则能显著延缓花粉活力的衰退。特别是在4 ℃的条件下,榴莲花粉保持了更高的活力。不同榴莲品种的花粉在贮藏期间活力变化差异显著,‘金枕’在所有贮藏条件下均表现出较强的活力维持能力。该研究结果为榴莲花粉贮藏技术的优化提供了理论依据。 |
| 英文摘要: |
| This study investigated the variations in pollen viability and stigma receptivity among four different durian (Durio zibethinus) cultivars. Using the benzidine-hydrogen peroxide method, stigma receptivity was quantitatively assessed, while changes in pollen viability under different storage conditions—namely room temperature (25?°C), 4?°C, and ?20?°C—were evaluated using the MTT staining method. The results revealed that stigma receptivity exhibited significant temporal fluctuations and a distinct developmental pattern across different flowering stages. Significant differences were also observed among cultivars, with ‘Monthong’ showing notably higher stigma receptivity compared to the other three cultivars. Regarding pollen storage, viability declined rapidly at room temperature and decreased significantly with prolonged storage time. In contrast, low-temperature storage at 4?°C and ?20?°C effectively delayed the decline in pollen viability, with 4?°C conditions proving particularly effective in preserving pollen vitality. Furthermore, the dynamics of pollen viability during storage varied significantly among cultivars, with ‘Monthong’ demonstrating the strongest capacity for maintaining viability under all tested conditions.These findings provide a theoretical basis for optimizing durian pollen storage techniques, contributing to improved reproductive management and breeding efficiency in durian cultivation. |
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