| 许婉祯,刘彩华,罗睿雄,高爱平,韦运谢,张贺.芒果类黄酮-3''-羟化酶（MiF3''H1）基因的鉴定与功能分析[J].中国南方果树,2026,55(4):86-93 |
| 芒果类黄酮-3''-羟化酶（MiF3''H1）基因的鉴定与功能分析 |
| Identification and Functional Analysis the Flavonoid-3''-Hydroxylase MiF3''H1 Gene from Mango |
| 投稿时间：2025-01-23 修订日期：2025-03-02 |
| DOI： |
| 中文关键词: 芒果 类黄酮-3''-羟化酶 花青素 叶绿素 功能分析 |
| 英文关键词:Mango Flavonoid-3''-hydroxylase Anthocyanin Aarotenoid Functional analysis |
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| 中文摘要: |
| 【目的】类黄酮-3''-羟化酶（Flavonoid-3''-Hydroxylase, F3''H）作为花青素合成的关键酶，在花青素的合成途径中至关重要。本文为了探究芒果（Mangifera indica）中MiF3''H1基因在果实着色的功能。【方法】本文对MiF3''H1进行克隆、生物信息学、原核表达和亚细胞定位分析，在番茄果实和芒果叶片进行过表达，并测定生理生化特征和相关合成酶基因的表达变化，综合分析MiF3''H1 在花青素生物合成中的功能。【结果】芒果MiF3''H1基因ORF全长1548 bp，编码515个氨基酸，通过系统进化树分析发现其与开心果F3''H的亲缘关系较近，MiF3''H1蛋白定位于细胞核和细胞质中。与对照组（空载体）相比，异源过表达MiF3''H1的番茄果实变红更早，且果实硬度小。同时，过表达MiF3''H1能够显著提高乙烯、脱落酸、花青素、类胡萝卜素、维生素C、莽草酸在芒果叶片和番茄果实的含量，且花青素、类胡萝卜素合成通路和叶绿素降解相关基因的表达量显著增高，加快叶绿素的降解。【结论】MiF3''H1具有促进乙烯、脱落酸、维生素 C 以及莽草酸的积累，这些物质的协同作用可加快芒果的成熟进程，同时参与颜色相关的花青素和类胡萝卜素的生物合成、加快叶绿素的降解，使芒果果实呈现不同的颜色，MiF3''H1可以作为选育早成熟且不同颜色品种的候选基因。 |
| 英文摘要: |
| 【Objective】Flavonoid-3''-hydroxylase, as the key enzyme in anthocyanin synthesis, plays crucial role in the synthesis pathway of anthocyanin. The objective of this study was explore the function of the MiF3''H1 gene at fruit coloration in mango (Mangifera indica).【Method】MiF3''H1 gene was cloned, bioinformatics analysis and subcellular localization analysis. Moreover, the function of MiF3''H1 in regulating anthocyanin biosynthesis was analysed via expression at tomato fruits and mango leaves with determined the physiological and biochemical characteristics and expression level of related-synthase genes.【Results】The results showed that the full-length ORF of the mango MiF3''H1 is 1548 bp, encoded 515 amino acids and the protein exhibited the closest relationship with F3''H from Pistacia vera. Simultaneously, subcellular localization showed that MiF3''H1was located in the both nucleus and cytosol in the leaf cells. Moreover, Solanum lycopersicum overexpression MiF3''H1 fruits exhibited red in earlier and less fruit hardness compared with the control (empty vector). Furthermore, overexpression MiF3''H1 at mango leaves and tomato fruits showed higher level of ethylene, abscisic acid, anthocyanins, carotenoids, vitamin C, shikimic acid, lower content of chlorophyll compared with the control. Correspondingly, the transcript level of genes related to anthocyanin-, carotenoid-synthesis pathways, and chlorophyll degradation gene was significantly increased in overexpression tissues. 【Conclusion】 Those results suggested that MiF3''H1 regulates mango fruit ripens via promoted the accumulation of ethylene, abscisic acid and vitamin C. Simultaneously, MiF3''H1 plays an important role in fruit color via promoted anthocyanin synthesis and chlorophyll degradation. MiF3''H1 could be used as a candidate gene for breeding early maturing and different colored varieties of mango. |
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