| 刘永志,云天艳,臧小平,井涛,陈伙干,李茂富,马蔚红.抗芒果炭疽病菌5-6菌株鉴定及拮抗机制研究[J].中国南方果树,2026,55(4):71-79 |
| 抗芒果炭疽病菌5-6菌株鉴定及拮抗机制研究 |
| Identification and antifungal mechanism of strain 5-6 against mango anthracnose |
| 投稿时间：2025-01-13 修订日期：2025-03-11 |
| DOI： |
| 中文关键词: 芒果炭疽病 生物防治 紫黑链霉菌 抑菌机理 广谱抑菌活性 |
| 英文关键词:Mango anthracnose biological control Streptomyces violaceusniger antifungal mechanism broad-spectrum antifungal activity |
| 基金项目:本研究由芒果品种科技示范园建设项目（2023-2025）；典型区域农业野生植物资源调查收集与监测等（合同编号：019240065）项目资助。 |
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| 中文摘要: |
| 【 目的 】明确拮抗菌5-6菌株的抑菌机理，为芒果炭疽病的生物防治提供理论基础和菌种资源。【 方法 】以芒果炭疽病菌（Colletotrichum gloeosporioides）为靶标菌，采用平板对峙法测定5-6菌株的抑菌活性；通过形态学、生理生化试验和基因组比对等方法确定5-6菌株的分类地位；利用扫描电子显微镜、酶活测定等方法，明确菌株5-6抗芒果炭疽病菌的抑菌机理。【 结果 】根据形态特征、生理生化特性和基因组比对信息，将菌株5-6归属为紫黑链霉菌（Streptomyces violaceusniger）。紫黑链霉菌5-6菌株对芒果炭疽病菌的抑菌活性为89.2%，其提取物的EC50值为65.73 μg/mL，能显著抑制芒果炭疽病菌菌体的增殖，且其提取物可导致病菌菌丝出现畸形、断裂、细胞膜受损、内含物溢出和抑制孢子萌发等现象，从而抑制病菌生长。菌株5-6能产生β-1,3葡聚糖酶、几丁质酶。同时菌株5-6还具有广谱抗真菌活性。【 结论 】芒果炭疽病拮抗菌菌株5-6鉴定为紫黑链霉菌。菌株5-6能产生葡聚糖酶、几丁质酶，其提取物能导致病菌细胞膜和孢子形态等受损，从而抑制病菌生长。因此，紫黑链霉菌5-6菌株是一株具有开发前景的生防微生物。 |
| 英文摘要: |
| 【Objective】To elucidate the antifungal mechanism of the antagonistic strain 5-6 against mango anthracnose, thereby providing a theoretical foundation and microbial resources for the biological control of mango anthracnose. 【Method】Targeting the mango anthracnose fungus (Colletotrichum gloeosporioides), we assessed the antibacterial activity of strain 5-6 using the dual culture method. The taxonomic status of 5-6 bacterial strains was determined through morphological, physiological, biochemical tests, and genomic comparison methods. To evaluate the antifungal mechanism of strain 5-6 against mango anthracnose by scanning electron microscopy and enzyme activity assays.【Results】Based on morphological, physiological, and biochemical characteristics, and genome alignment information, strain 5-6 was identified as Streptomyces violaceusniger. This Streptomyces violaceusniger 5-6 strain exhibited an antifungal activity of 89.2% against mango anthracnose, with an EC50 value of 65.73 μg/mL for its extract. This extract significantly inhibited the proliferation of the mango anthracnose pathogen, causing malformations, breakages, cell membrane damage, content overflow, and spore germination inhibition, thereby suppressing pathogen growth. Strain 5-6 is capable of producing β-1,3 glucanase and chitinase. Additionally, this strain exhibits broad-spectrum antifungal activity. 【Conclusion】The antagonistic strain 5-6 against mango anthracnose was identified as Streptomyces violaceusniger. Strain 5-6 is capable of producing glucanase and chitinase, and its extract can damage the cell membrane and spore morphology of the pathogen, thereby inhibiting its growth. Therefore, Streptomyces purpureus 5-6 is a promising biocontrol microorganism.
Keywords：Mango anthracnose; biological control; Streptomyces violaceusniger; antifungal mechanism; broad-spectrum antifungal activity |
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