| 何斌,刘仕丽,叶佳成,唐大海,刘林娅,黄亚成.‘红阳‘猕猴桃RuBisCO小亚基基因 (AcRbcS) 的克隆与表达分析[J].中国南方果树,2026,55(1): |
| ‘红阳‘猕猴桃RuBisCO小亚基基因 (AcRbcS) 的克隆与表达分析 |
| Cloning and Expression Analysis of a RuBisCO small subunit gene (AcRbcS) in Actinidia chinensis cv. Hongyang |
| 投稿时间：2024-11-14 修订日期：2025-01-24 |
| DOI：10.13938/j.issn.1007-1431.20240558 |
| 中文关键词: ‘红阳’猕猴桃 RuBisCo小亚基 基因克隆 实时荧光定量PCR |
| 英文关键词:Actinidia chinensis cv. Hongyang RbcS Gene cloning QPCR |
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| 中文摘要: |
| 【目的】 本研究旨在克隆‘红阳’猕猴桃（Actinidia chinensis cv. Hongyang）中编码核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCo）小亚基的基因（命名为AcRbcS），并分析其表达模式及生物学功能，为提高‘红阳’猕猴桃光合生产力的定向育种提供理论依据。【方法】 通过基因克隆技术从‘红阳’猕猴桃中获得AcRbcS基因，并利用RNA-seq和实时荧光定量PCR技术分析其在叶片不同发育时期的表达模式。【结果】 AcRbcS基因CDS全长369 bp，编码122个氨基酸残基，蛋白分子量为13.75 kDa。表达分析显示，AcRbcS在‘红阳’猕猴桃叶片发育过程中表达丰度随发育时期增加而上升，且在淡绿期达到峰值。【结论】 研究结果表明，AcRbcS基因在‘红阳’猕猴桃光合作用中具有重要作用，其高表达与光合效率密切相关。该研究为后续通过分子育种手段提高‘红阳’猕猴桃光合作用效率和果实品质提供了重要参考。 |
| 英文摘要: |
| [Objective] This study aims to clone the gene encoding the small subunit of Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) from ‘Hongyang’ kiwifruit (Actinidia chinensis cv. Hongyang), designated as AcRbcS. The expression pattern and biological function of this gene were analyzed to provide a theoretical basis for targeted breeding strategies aimed at enhancing the photosynthetic productivity of ‘Hongyang’ kiwifruit. [Methods] The AcRbcS gene was cloned from ‘Hongyang’ kiwifruit using molecular techniques. Its expression profile across different leaf developmental stages was investigated using RNA-seq and real-time quantitative PCR (qPCR). [Results] The coding sequence (CDS) of AcRbcS is 369 bp in length, encoding a protein of 122 amino acids with a molecular weight of 13.75 kDa. Expression analysis revealed that the abundance of AcRbcS mRNA increased progressively during leaf development, reaching a peak at the light-green stage. [Conclusion] The findings demonstrate that AcRbcS plays a significant role in the photosynthetic process of ‘Hongyang’ kiwifruit, with its elevated expression being closely associated with enhanced photosynthetic efficiency. This study provides valuable insights for future molecular breeding efforts to improve the photosynthetic efficiency and fruit quality of ‘Hongyang’ kiwifruit. |
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