| 黄小丽,周丽容,郑雪文,任小玉,冯学杰,陈迪,何小龙,徐光照,杨转英.榴莲蔗糖合成酶基因家族的挖掘与表达模式分析[J].中国南方果树,2025,54(6): |
| 榴莲蔗糖合成酶基因家族的挖掘与表达模式分析 |
| Excavation and Expression Pattern Analysis of Durian Sucrose Synthase Gene Family |
| 投稿时间：2024-08-14 修订日期：2024-09-22 |
| DOI：10.13938/j.issn.1007-1431.20240425 |
| 中文关键词: 榴莲 蔗糖合成酶（SUS） 生物信息学 表达分析 |
| 英文关键词:Durian Sucrose Synthase（SUS） Bioinformatics Expression Analysis |
| 基金项目:基金资助：阳江市榴莲引种及设施化高效优质栽培技术（SDZX2022027） |
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| 中文摘要: |
| 蔗糖合成酶（Sucrose Synthase，SUS）参与植物糖分转运、分配及合成，对果实品质形成和产量至关重要。本研究通过分析榴莲中蔗糖合成酶基因结构、系统进化关系和表达特性等，旨在初步鉴定其功能。结果表明，1）从榴莲基因组中鉴定出8个SUS基因家族成员，命名为DzSUS1～DzSUS8，均呈弱酸性、亲水性，主要分布于细胞膜中。分子量大小在92031.51～105181.90d范围内，编码氨基酸805～931个。2）二级结构均由α-螺旋、延伸链、β-转角和无规则卷曲等四种模式构成，其中α-螺旋结构比例最高。3）DzSUS基因结构相似，外显子为13～15个，内含子12～14个，但基因全长存在显著性差异，最长的是DzSUS6基因，最短的是DzSUS1。DzSUS蛋白均含有10个保守基序，具有高度保守性。4）DzSUS与黄褐棉、亚洲棉、达尔文棉、拟南芥、木槿SUS的亲缘关系较近，大致分为两大类，DzSUS4与DzSUS5为Ⅰ类，DzSUS1、DzSUS2、DzSUS3、DzSUS6、DzSUS7、DzSUS8为Ⅱ类。5）榴莲果实中蔗糖含量显著高于葡萄糖和果糖，为典型蔗糖积累型；不同品种糖组分不同，DzSUS1与果糖、葡萄糖呈现显著正相关，DzSUS7与总糖、蔗糖含量呈显著负相关，推测DzSUS1和DzSUS7可能参与榴莲果实糖分积累。通过对榴莲DzSUS基因生物信息学及表达分析，将为研究其生理功能和调控机制奠定了理论基础。 |
| 英文摘要: |
| Sucrose Synthase (SUS) plays a crucial role in plant sugar transport, distribution, and synthesis, which is vital for fruit quality formation and yield. This study aimed to preliminarily identify the functions of SUS genes in durian by analyzing their gene structure, phylogenetic relationships, and expression characteristics. Results indicated that: 1) Eight SUS gene family members were identified from the durian genome, named DzSUS1 to DzSUS8, all of which are weakly acidic and hydrophilic, primarily localized in the cell membrane. The molecular weight ranges from 92031.51 to 105181.90d, encoding 805 to 931 amino acids. 2) The secondary structures consist of α-helices, extended chains, β-turns, and unordered coils, with the highest proportion of α-helices. 3) The gene structures of DzSUS are similar, with 13 to 15 exons and 12 to 14 introns, but there are significant differences in gene length, with the longest being DzSUS6 and the shortest DzSUS1. DzSUS proteins all contain 10 conserved motifs, showing high conservation. 4) DzSUS have a close phylogenetic relationship with SUS from Gossypium hirsutum, Gossypium arboreum, Gossypium darwinii, Arabidopsis thaliana, and Hibiscus syriacus, roughly divided into two major categories, with DzSUS4 and DzSUS5 as Class I, and DzSUS1, DzSUS2, DzSUS3, DzSUS6, DzSUS7, and DzSUS8 as Class II. 5) The sucrose content in durian fruit is significantly higher than that of glucose and fructose, characteristic of a typical sucrose-accumulating fruit; different varieties have different sugar compositions, with DzSUS1 showing a significant positive correlation with fructose and glucose, while DzSUS7 showing a significant negative correlation with total sugar and sucrose content, suggesting that DzSUS1 and DzSUS7 may be involved in sugar accumulation in durian fruit. The bioinformatics and expression analysis of durian DzSUS genes provide a theoretical foundation for studying their physiological functions and regulatory mechanisms. |
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