| 罗义灿,邓有展,姚金洁,吴凤,秦玉燕,刘力,王运儒.基于HS-SPME-GC-MS的不同砧木成熟期沃柑香气成分分析[J].中国南方果树,2025,54(5): |
| 基于HS-SPME-GC-MS的不同砧木成熟期沃柑香气成分分析 |
| Analysis of Aromatic Compounds in Ripening Orah mandarin (Citrus reticulata Blanco) on Different Rootstocks Using HS-SPME-GC-MS |
| 投稿时间：2024-07-15 修订日期：2024-08-29 |
| DOI：10.13938/j.issn.1007-1431.20240379 |
| 中文关键词: 沃柑 砧木 香气成分 气相色谱-质谱联用 生物标志物 |
| 英文关键词:Orah mandarin rootstock aromatic components Gas chromatography-mass spectrometry (GC-MS) biomarker |
| 基金项目:广西自然科学基金青年项目(2021GXNSFBA196006)；国家现代农业产业技术体系广西柑橘创新团队“采后技术与质量安全”岗位功能专家(nycytxgxcxtd-2021-05-03)；广 西农业科学院基本科研业务专项农产品质量安全风险评估研究(桂农科 2021YT147) |
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| 中文摘要: |
| 为探明不同砧木沃柑成熟期果实香气成分的差异性，对以酸橘、香橙和枳壳等3种为砧木的27个沃柑果肉样品进行HS-SPME-GC-MS分析，共鉴定出60种香气成分，包括烯烃、醇、醛、酮、烷和酯类等，其中D-柠檬烯为最主要的香气成分。通过PCA和OPLS-DA模型分析，发现了不同砧木沃柑在香气成分上的生物潜在标志物。D-柠檬烯、β-月桂烯、正辛醛和（2Z）-3-（3，4-二甲氧基苯基）-2-（3-吡啶基）丙烯腈是区分酸橘和香橙香气潜在生物标志物；D-柠檬烯、β-月桂烯、正辛醛和正辛醇是酸橘和枳壳的香气潜在生物标志物；正辛醛、Z)-3,7-二甲基-1,3,6-十八烷三烯、正辛醇、（2Z）-3-（3，4-二甲氧基苯基）-2-（3-吡啶基）丙烯腈、(-)-α-蒎烯和萜品油烯是区分酸橘和香橙香气潜在生物标志物；正辛醛是区分3种不同砧木的香气潜在生物标志物。研究结果为沃柑砧木选择和品质改良提供了科学依据。 |
| 英文摘要: |
| To elucidate the differences in the aromatic composition of citrus fruits at maturity when grafted onto different rootstocks, 27 pulp samples of citrus fruits grafted onto three rootstocks, including sour orange, sweet orange, and trifoliate orange, were analyzed using HS-SPME-GC-MS. A total of 60 volatile compounds were identified, encompassing alkenes, alcohols, aldehydes, ketones, alkanes, and esters, with D-limonene being the predominant aromatic compound. Through PCA and OPLS-DA model analysis, potential biomarkers for the aroma of citrus fruits grafted onto different rootstocks were discovered. D-limonene, β-myrcene, n-octanal, and (2Z)-3-(3,4-dimethoxyphenyl)-2-(3-pyridyl)acrylonitrile were identified as potential biomarkers distinguishing the aroma of citrus fruits grafted onto sour orange and sweet orange rootstocks; D-limonene, β-myrcene, n-octanal, and n-octanol were potential biomarkers for the aroma of citrus fruits grafted onto sour orange and trifoliate orange rootstocks; n-octanal, (Z)-3,7-dimethyl-1,3,6-octatriene, n-octanol, (2Z)-3-(3,4-dimethoxyphenyl)-2-(3-pyridyl)acrylonitrile, (-)-α-pinene, and terpinolene were potential biomarkers distinguishing the aroma of citrus fruits grafted onto sour orange and sweet orange rootstocks; n-octanal was a potential biomarker for the aroma of citrus fruits grafted onto all three different rootstocks. The findings provide a scientific basis for the selection of rootstocks and the improvement of citrus fruit quality. |
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