| 栗现芳,李晓娟,刘锦峰,金波,陈国梁,王建华,王延峰.基于木枣变异系枣裂果相关基因的鉴定[J].中国南方果树,2024,53(6): |
| 基于木枣变异系枣裂果相关基因的鉴定 |
| Identification of Genes Related to Dehiscent Fruit in Jujube Based on Muzao Variation Lines |
| 投稿时间：2024-06-23 修订日期：2024-07-16 |
| DOI： |
| 中文关键词: 枣 裂果 转录组 基因表达 |
| 英文关键词:Jujube Dehiscent fruit Transcriptome Gene expression |
| 基金项目:国家自然科学基金项目（31860535）；陕西省科技计划项目（2020JM-553，2021JM-415）；陕西省教育厅重点实验室项目（18JS118）；延安市科技计划项目（2018KN-01-01）；陕西省大学生创新创业训练计划项目（S202110719121）和延安大学校级大学生创新创业训练计划项目（D2021124）；横向项目（206020872） |
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| 中文摘要: |
| 枣裂果现象普遍严重，不同品种枣果抗裂性明显不同。本研究以‘木枣’未裂果、抗裂变异系‘V2’未裂果及木枣裂果为材料，通过RNA-seq技术构建枣抗裂相关转录本文库，并利用qRT-PCR技术进行表达验证。结果表明：RNA-seq共得到69.6G的原始数据，Q30达到92.7%以上，GC含量平均值为45.1%，各样品Reads与参考基因组的比对效率在79.82%-85.81%之间。‘木枣’未裂果及‘V2’未裂果间筛选出50个差异表达基因，26个基因表达上调、24个基因表达下调。‘木枣’未裂果与裂果间筛选出12个差异表达基因，8个基因表达上调、4个基因表达下调。GO富集分析分别有49、12个差异基因获得了功能注释，生物学过程在品种抗裂性中发挥重要作用。KEGG富集分析差异基因主要分布在代谢相关通路，尤其是淀粉和糖代谢、嘌呤和嘧啶代谢，个别分类在遗传信息处理通路，且都未被分类在细胞过程、环境信息处理过程等通路。qRT-PCR表达验证，PHD finger蛋白、纤维素合成酶D5、 bc1复合蛋白激酶、羧酸酯酶等基因差异表达显著，可进行后续基因功能验证研究。 |
| 英文摘要: |
| The fruit cracking of jujube is generally serious, and the cracking resistance of different varieties is obviously different. In this study, the transcriptional library related to crack resistance was constructed and the key genes were screened by RNA-seq and qRT-PCR technology used the uncracked fruit of 'Muzao', the uncracked fruit of 'V2' and the cracked fruit of 'Muzao'. The results show that a total of 69.6G of original data was obtained, Q30 reached more than 92.7%, the GC content was 45.1%, the alignment efficiency of Reads between each sample and reference genome ranged from 79.82% to 85.81% by RNA-seq. 50 differentially expressed genes were screened between the uncracked fruit of 'Muzao' and the uncracked fruit of 'V2', among which 26 genes were up-regulated and 24 genes were down-regulated. 12 differentially expressed genes were screened between uncracked and cracked fruit of 'Muzao', among which 8 genes were up-regulated and 4 genes were down-regulated. GO analysis showed that 49 and 12 differential genes were functionally annotated respectively and the biological process play an important role in crack resistance of cultivars. KEGG enrichment analysis showed that most of the differential genes were distributed in metabolism-related pathways, especially starch and sucrose metabolism, purine and pyrimidine metabolism,some genes were classified in genetic information processing, and none of them were classified in cell processing and environmental information processing. qRT-PCR showed that the PHD finger protein, cellulose synthase-like protein D5, protein activity of BC1 complex kinase and carboxylesterase genes were significantly differentially expressed, and could be used for subsequent gene functional validation studies. |
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