| 王维凤,范建新,陈七艳,潘国追.黔东南24份杨梅资源的遗传多样性分析[J].中国南方果树,2025,54(6): |
| 黔东南24份杨梅资源的遗传多样性分析 |
| Genetic Diversity Analysis of 24 Bayberry Resources in Southeastern Guizhou |
| 投稿时间：2024-06-11 修订日期：2024-08-16 |
| DOI： |
| 中文关键词: 杨梅 遗传多样性 种质资源 SRAP |
| 英文关键词:Bayberry Genetic diversity Germplasm resources SRAP |
| 基金项目:凯里学院大学生创新创业训练计划项目（黔东南杨梅资源的遗传多样性分析，编号：X202210669008）；贵州省高等学校贵州山地特色园艺作物分子育种与品种创制重点实验室（黔教技[2022]053号）；凯里学院博士启动项目（BS20220101） |
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| 中文摘要: |
| 为了探究黔东南杨梅资源的亲缘关系，了解其遗传距离远近，为后续杨梅良种认定与杨梅品种选育及合理选配亲本提供参考。以黔东南州24份杨梅资源为材料，基于SRAP分子标记技术分析黔东南杨梅资源的遗传多样性。结果表明：10对引物扩展出的条带多态性比率为95.7%；观察到平均观测等位基因数、有效等位基因数、Nei’s基因多样性指数、Shannon信息指数分别为1.3826、1.2432、0.1397、0.2079。得到6个种群间的基因分化系数Gst=0.1232，表明总变异中有19.31%的变异存在于种群间；得到6个种群间的基因流Nm=2.4556；杨梅种质6个种群的平均遗传距离为0.2460，平均遗传相似度为0.7869；从24份杨梅资源的聚类树状图上可以明显看出栽培品种与野生种质分开聚类，4号野生种质与第一大类的栽培品种聚为一类，可将其作为关键的育种材料，为增加杨梅遗传多样性提供更多可能。黔东南杨梅种质有着较为丰富的遗传多样性，但黔东南野生杨梅资源的亲缘关系与地理来源相关性不甚明显。 |
| 英文摘要: |
| In order to explore the genetic relationship of Bayberry resources in southeastern Guizhou, understand their genetic distance, and provide reference for the subsequent identification of Bayberry varieties, selection of Bayberry varieties, and rational selection of parents. Using 24 Bayberry resources from Qiandongnan Prefecture as materials, the genetic diversity of Bayberry resources in Qiandongnan was analyzed based on SRAP molecular marker technology. The results showed that the polymorphism rate of the bands extended by 10 pairs of primers was 95.7%; The average observed number of alleles, effective number of alleles, Nei's gene diversity index, and Shannon information index were observed to be 1.3826, 1.2432, 0.1397, and 0.2079, respectively. The gene differentiation coefficient Gst=0.1232 was obtained among 6 populations, indicating that 19.31% of the total variation exists between populations; Obtain gene flow Nm=2.4556 among 6 populations; The average genetic distance of the six populations of Bayberry germplasm is 0.2460, and the average genetic similarity is 0.7869; From the clustering tree diagram of 24 Bayberry resources, it can be clearly seen that cultivated varieties and wild germplasm are clustered separately. Wild germplasm 4 is clustered together with the cultivated varieties in the first major category, which can be used as a key breeding material to provide more possibilities for increasing Bayberry genetic diversity. The germplasm of Bayberry in Qiandongnan has a relatively rich genetic diversity, but the genetic relationship and geographical origin correlation of wild Bayberry resources in Qiandongnan are not very obvious. |
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