| 张莉娟,林垠孚,陆覃昱,莫干辉,苏文潘,吴凤.基于外壳蛋白基因的广西西番莲夜来香花叶病毒的遗传多样性分析[J].中国南方果树,2025,54(5): |
| 基于外壳蛋白基因的广西西番莲夜来香花叶病毒的遗传多样性分析 |
| Genetic diversity analysis of Telosma mosaic virus isolates from passion fruit in Guangxi based on coat protein gene |
| 投稿时间：2024-05-09 修订日期：2024-06-18 |
| DOI：10.13938/j.issn.1007-1431.20240255 |
| 中文关键词: 广西 西番莲 TeMV CP基因 遗传多样性 遗传变异 |
| 英文关键词:Guangxi passion fruit TeMV CP gene Genetic diversity Genetic variation |
| 基金项目:广西农业科学院基本科研业务专项（桂农科2022YM14） |
|
| 摘要点击次数: 184 |
| 全文下载次数: 0 |
| 中文摘要: |
| 摘要：为明确夜来香花叶病毒（Telosma mosaic virus，TeMV）在广西地区的发生情况和群体遗传特征，对采集自广西17个西番莲产区的187份疑似感染病毒的西番莲样品进行RT-PCR检测，并选取来自不同产区样品的外壳蛋白（coat protein，CP）基因进行克隆和测序，分析其系统发育、遗传进化、种群结构等特征。结果表明：187份样品共有162份检出TeMV，检出率为86.6%。共获得22个TeMV CP基因序列（GenBank登录号：OR786355-OR786369，PP544171-PP544177）。相似性结果表明，22个西番莲TeMV分离物CP基因的核苷酸序列一致性为94.7%-100%，氨基酸序列一致性为95.7%-100%。结合GenBank下载的19条序列的系统发育分析表明，41条序列分为3个大的分支，广西西番莲TeMV分离物属于I组；不同国家或地区来源的分离物相互分离，且相同寄主来源的分离物亲缘关系最近。西番莲TeMV分离物CP基因序列无重组现象。进化的主要驱动力是负选择压力。不同组间的遗传分化显著，基因交流频率低，易发生遗传漂变，群体处于扩张趋势。本研究是针对广西大部分地区西番莲上TeMV遗传变异的首次报道，研究结果为广西西番莲对TeMV的防控提供参考。 |
| 英文摘要: |
| Abstrat: To investigate the incidence and genetic diversity of Telosma mosaic virus (TeMV) on passion fruit in Guangxi , a total of 187 samples suspected to be infected with viruses were collected from 17 passion fruit producing areas in Guangxi. These samples were subsequently detected using RT-PCR. Phylogenetic analysis, genetic evolution, and population structure were analyzed based on coat protein (CP) genes sequence obtained from samples collected across different production areas. The results revealed that TeMV was detected in 162 out of 187 samples with a detection rate of 86.6%. A total of 22 TeMV CP gene sequences were sequenced. Similarity analysis demonstrated that the nucleotide and amino acid sequences were 94.7%-100% and 95.7%-100% respectively. Phylogenetic analysis combining the obtained CP sequences with an additional 19 TeMV CP sequences retrieved from GenBank showed that 41 sequences were divided into three major branches. All TeMV isolates generated in this study belonged to group I. Isolates originating from different geographical regions clustered in distinct lineages, while isolates from the same host species were most closely related. No recombination event in 41 CP gene sequences were observed. Negative selection was identified as the primary driving force behind TeMV evolution observed here. The genetic differentiation among different groups was statistically significant, indicating a low frequency of gene exchange and a propensity for genetic drift to occur. Furthermore, the population was in the trend of expansion. This study is the first to report the genetic diversity of TeMV in majority area of Guangxi passion fruit, and the results of provide reference for the control of TeMV in passion fruit. |
| 查看/发表评论 下载PDF阅读器 |
| 关闭 |
