| 王莹,王倩,邵远志,李雯.杧果MiCLH1基因的时空表达模式分析[J].中国南方果树,2025,54(4): |
| 杧果MiCLH1基因的时空表达模式分析 |
| Temporal and spatial expression analysis of the MiCLH1 gene in mango |
| 投稿时间：2024-04-28 修订日期：2024-08-30 |
| DOI：10.13938/j.issn.1007-1431.20240220 |
| 中文关键词: 关键词：芒果果皮 MiCLH1基因 生物信息学 表达模式 |
| 英文关键词:Key words: Mango peel MiCLH1 gene Bioinformatics Expression pattern |
| 基金项目:海南省院士团队创新中心建设项目（SQ2021YSPTJXRWS0074）；国家自然科学基金面上项目（No. 32072275) |
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| 中文摘要: |
| 摘 要：叶绿素酶（Chlorophyllase，CLH）是叶绿素分解代谢途径中的关键酶。本研究以‘台农一号’杧果（Mangifera indica L.）为材料，分析了MiCLH1基因的生物学特征，并采用荧光定量PCR方法检测不同采后处理（1μL/L 1-甲基环丙烯和5g/L Ethylene）杧果果皮中MiCLH1基因的表达模式。结果表明：MiCLH1全长960bp，编码320个氨基酸，等电点为5.62，蛋白质分子量为34.08kD。序列比对和进化树分析表明，MiCLH1与阿月浑子同源性最高。启动子序列分析显示，MiCLH1基因的启动子序列中激素响应元件含有乙烯响应元件。对杧果果皮叶绿素和类胡萝卜素含量进行测定，结果表明1-MCP处理能够延缓采后杧果果皮叶绿素的降解，ETH处理能够促进采后杧果果皮叶绿素的降解，进一步的RT-qPCR 分析表明，ETH处理促进了MiCLH1的表达，1-MCP处理抑制了MiCLH1的表达。 |
| 英文摘要: |
| Abstract: Chlorophyllase (CLH) is a key enzyme in chlorophyll catabolism pathway. In this study, the biological characteristics of MiCLH1 gene were analyzed by using 'Tainong No. 1' mango as material, and the expression patterns of MiCLH1 gene in different postharharvest treatments (1μL/L 1-MCP and 5g/L ETH) were detected by fluorescence quantitative PCR. The results show that MiCLH1 is 960bp in length, has a complete open reading frame, encodes 320 amino acids, has an isoelectric point of 5.62, and its molecular weight is 34.08kD. Sequence alignment and evolutionary tree analysis showed that MiCLH1 and pistachio had the highest homology, and the amino acid sequence similarity reached 81.05%. Promoter sequence analysis showed that the promoter of MiCLH1 gene contained photoresponsive elements, plant disease response elements, transcription factor response elements and hormone response elements, among which the number of photoresponsive elements was more than other elements, the hormone response elements included ethylene response elements and abscisic acid response elements, suggesting that MiCLH1 protein was located in the cytoplasm. The content of chlorophyll and carotenoid in mango peel was determined. The results showed that both 1-MCP and ETH treatments could significantly affect the color transformation of mango after harvest. Further RT-qPCR analysis showed that ethylene treatment could significantly up-regulate the expression of MiCLH1 gene, while 1-MCP could significantly down-regulate the expression of MICLH1 gene. The changes of chlorophyll enzyme activity and gene expression levels in each treatment showed the same trend, indicating that MiCLH1 gene plays a key role in chlorophyll degradation in mango peel. This study laid a foundation for further exploring the regulatory network of ethylene on chlorophyll degradation of mango and improving the color transformation of mango in production. |
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