| 黄伟,柴爽,张犇,龚玲,王双友,李大志,龙桂友,李娜.“早蜜椪柑”杂交组合的胚培养及杂种鉴定[J].中国南方果树,2025,54(2): |
| “早蜜椪柑”杂交组合的胚培养及杂种鉴定 |
| Embryo Culture and Hybrid Identification of Zaomi Ponkan Hybrid Combinations |
| 投稿时间：2024-03-27 修订日期：2024-04-17 |
| DOI： |
| 中文关键词: 柑橘 早蜜椪柑 胚培养 培养基 杂种鉴定 |
| 英文关键词:citrus embryo rescue in vitro culture medium hybrid identification |
| 基金项目:国家柑橘产业技术体系项目(CARS-26)；湖南省重点研发计划(2022NK2015)资助和湖南省水果产业技术体系柑橘育种岗位专项和资助。 |
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| 中文摘要: |
| 为了培育出具有红色果皮或果肉的椪柑新品种,本研究以本地早熟品种“早蜜椪柑”为母本,“南橘”、“阿弗勒”和“沃柑”为父本进行杂交,配置不同萌发培养基来确定最适培养基中蔗糖和GA3浓度,对授粉后不同时期的幼胚分别进行培养,最后利用SSR标记对杂交后代进行鉴定。结果表明：MT+7%蔗糖+GA3 1.0 mg/L适合幼胚培养；三个杂交组合后代成苗率最高是在授粉后100 d的幼胚；通过SSR分子标记鉴定得到“早蜜椪柑”ד南橘”杂种苗48株,“早蜜椪柑”ד阿弗勒”杂种苗1株。 |
| 英文摘要: |
| In order to cultivate a new variety of ponkan with red peel or pulp, the present study used the local early-maturing variety "Zaomi Ponkan Nanju Afourer 3 in the medium by configuring different germination media, cultured the young embryos at different stages after pollination, and finally used SSR markers to identify the hybrid offspring. The results showed that MT+7% sucrose+GA3 1.0 mg/L was suitable for young embryo culture, the highest seedling formation rate of the three hybrid combinations was the young embryo 100 days after pollination, and 48 hybrid seedlings of "Zaomi Ponkan”דNanju Zaomi Ponkan”× “Afleur” were identified by SSR molecular marker method |
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