| 席培宇,吴凤婵,郭玉琳,李安定.百香果茎段组织培养及其脱毒快繁体系的建立[J].中国南方果树,2025,54(2): |
| 百香果茎段组织培养及其脱毒快繁体系的建立 |
| Study on Tissue Culture and Virus-free Technology of Stem Segment of Passion Fruit |
| 投稿时间：2024-02-02 修订日期：2024-03-12 |
| DOI：10.13938/j.issn.1007-1431.20240071 |
| 中文关键词: 百香果 茎段 组织培养 脱毒 RT-PCR |
| 英文关键词:Passion Fruit Stem segment tissue culture detoxification RT-PCR |
| 基金项目: |
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| 摘要点击次数: 1063 |
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| 中文摘要: |
| 以紫果百香果台农“1”号的嫩茎为外植体，改良的RMS添加7g/L的琼脂、25g/L的蔗糖和0.5g/L花宝1号为基本培养基，通过添加不同激素直接诱导腋芽萌发成苗培养，再采用热处理结合茎尖培养进行脱毒处理，得到的组培苗经过病毒检测后，未发现百香果黄瓜花叶病毒(CMV)、西番莲木质化病毒（PWV）、西番莲斑驳病毒(PaMV)、西番莲扭叶病毒（PLDV）和东亚西番莲病毒(EAPV)。选取病毒检测阴性组培苗进行芽增殖及生根培养，优化不同培养阶段最佳的激素种类、浓度和配比，集成了紫果百香果的脱毒快繁体系，为实现紫果百香果脱毒种苗的快速繁殖及工厂化生产提供技术支撑。结果表明：RMS+2.5mg/L ZEA适合丛生芽诱导，诱导率达96%；RMS+1.5mg/L ZEA +0.2mg/L NAA+0.1mg/L GA3适合继代增殖培养，增殖系数达3.43；RMS+1.0mg/L IBA+0.2mg/L NAA适合生根诱导，生根率达100%。采用热处理结合茎尖培养得到的组培苗经过病毒复检后发现，20份样品都未检测出CMV、PWV、PaMV、PLDV和EAPV，5种病毒的脱毒率达100%，突破了百香果脱毒种苗的快速繁育技术瓶颈。 |
| 英文摘要: |
| Using the tender stems of passion fruit Tainong "1" as explants,the modified RMS added 7 g/L agar,25 g/L sucrose and 0.5 g/L Huabao 1 as basic medium,the axillary buds were directly induced to germinate by adding different hormones,and the plantlets were cultured by heat treatment combined with stem tip culture,after virus detection, no passion fruit cucumber Mosaic virus (CMV), passion fruit lignification virus (PWV), passion fruit mottling virus (PaMV), passion fruit leaf twist virus (PLDV) or East Asian passion fruit virus (EAPV) were found in the tissue culture seedlings.The bud multiplication and rooting culture of tissue culture plantlets with negative virus detection were carried out to optimize the optimum hormone species,concentration and proportion in different culture stages,to provide technical support for rapid propagation and industrial production of virus-free seeds of passion fruit. The results showed that RMS+2.5mg/L ZEA was suitable for inducing clustered buds, with an induction rate of 96%;RMS+1.5mg/L ZEA+0.2mg/L NAA+0.1mg/L GA3 is suitable for subculture proliferation culture,with a proliferation coefficient of 3.43;RMS+1.0mg/L IBA+0.2mg/L NAA is suitable for rooting induction, with a rooting rate of 100%. After virus detection,it was found that 20 samples of tissue cultured seedlings obtained through heat treatment combined with stem tip culture did not detect CMV,PWV,PaMV,PLDV and EAPV.The detoxification rate of the 5 viruses reached 100%, breaking through the bottleneck of rapid breeding technology for virus-free passion fruit seedlings. |
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