| 滕少娜.柑桔溃疡病菌PCR检测方法效果评价[J].中国南方果树,2024,53(6): |
| 柑桔溃疡病菌PCR检测方法效果评价 |
| Evaluation of PCR detection methods for Xanthomonas citri pv.citri |
| 投稿时间：2023-12-11 修订日期：2023-12-13 |
| DOI：10.13938/j.issn.1007-1431.20230604 |
| 中文关键词: 柑桔溃疡病菌 检测 引物 常规PCR 实时荧光PCR |
| 英文关键词:Xanthomonas citri pv.citri detection primer Conventional PCR Real-time PCR |
| 基金项目:重庆市科技局项目 |
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| 中文摘要: |
| 为准确检测柑桔溃疡病菌，减少检测过程中假阳性和假阴性结果，为农业执法部门和口岸一线检疫鉴定提供参考。本研究采用已报道的14对引物/探针，对来自不同国家和地区的22株柑桔溃疡病菌及相关菌株进行了特异性测试和灵敏度比对分析，并测试了具疑似柑桔溃疡病症状的实际样品9份。测试结果显示，引物JYF5(XccF05)/JYR5(XccR05)和Xac01/Xac02的检测特异性高，其它12对引物/探针会出现不同程度上的假阳性或假阴性结果；两对引物检测的灵敏度均为3 pg/μL；引物XAcF/XAcR对来自泰国的青柠样品和阳性菌株，检测均为假阴性。根据测试结果，推荐这2对引物用于柑桔溃疡病菌的检测，优先推荐引物JYF5(XccF05)/JYR5(XccR05)。 |
| 英文摘要: |
| In order to accurately detect citrus bacteria canker, reduce false positive and false negative results in the detection process, and provide reference for agricultural law enforcement departments and port quarantine identification. In this study, 14 pairs of primers/probes were used to analyze the specificity and sensitivity of 22 strains and related strains from different countries and regions, and 9 samples with suspected symptoms of citrus canker were tested. The test results showed that primers JYF5(XccF05)/JYR5(XccR05) and Xac01/Xac02 had high specificity, and the other 12 primers/probes had false positive or false negative results to varying degrees. The sensitivity of the two primers was 3 pg/μL. Primer XAcF/XAcR tested false negative for lime samples and positive strains from Thailand. According to the test results, these two pairs of primers are recommended for the detection of citrus ulcer bacteria, and the primers JYF5(XccF05)/JYR5(XccR05) are preferred. |
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