| 王丽萍,邓泽周,吴勇谋,田利华,谢健,谢晓辉,张和顺,梁番土.皇帝蕉(Musa AA)组织培养与快速繁殖技术研究[J].中国南方果树,2014,43(2): |
| 皇帝蕉(Musa AA)组织培养与快速繁殖技术研究 |
| Study of the technique of fast propagation and tissue culture for Musa AA |
| 投稿时间：2013-11-13 修订日期：2013-12-10 |
| DOI： |
| 中文关键词: 皇帝蕉 组织培养 快速繁殖 |
| 英文关键词:Musap AA tissue culture fast propagation |
| 基金项目: |
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| 中文摘要: |
| 摘要：对皇帝蕉芽的消毒方法、外植体的诱导、幼芽的增殖培养、生根培养等进行了研究。结果表明：采用75%酒精60s--0.1%升汞12min（第一次8min 第二次4 min）的消毒方法，比较适宜于皇帝蕉外植体的消毒，易获得理想的无菌体系；在芽的诱导初期阶段，6-BA浓度采用5.0 mg/L，最适合皇帝蕉外植体的诱导；在幼芽的中高代增殖培养阶段，适宜采用MS 4.0mg/L6-BA 0.1mg/LNAA配方；在幼芽的高代增殖培养阶段，适宜采用MS 3.0mg/L6-BA 0.1mg/LNAA配方；在幼芽的生根阶段，适宜采用MS 2.5mg/LIBA 0.2mg/LNAA配方，幼苗生根快、根系壮实、发达，成活率高。 |
| 英文摘要: |
| Abstract: It were studied that sterilize method、induction of explant and multiplication culture and rooting culture for Musa AA bud .The results indicated the sterile treatment of 75% alcohol for 60 seconds, 0.1% corrosive sublimate for 12 min(first 8 min second time 4 min) is more appropriate in sterilize of explants for Musap AA，It was easy to obtain ideal aseptic system；The concentration of 6-BA was 5 mg/L, the most suitable for the induction of Musap AA explants in the early stages of bud induction； It was suitable for MS 4.0mg/L6-BA 0.1mg/LNAA formula in the stage of culture middle and high proliferation for bud；It was suitable for MS 3.0mg/L6-BA 0.1mg/LNAA formula In the stage of culture high proliferation for bud ；It was suitable for MS 2.5mg/LIBA 0.2mg/LNAA formula in shoots rooting stage of bud, Seedling root quickly, root was stout and developed , survival rate was high. |
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